Core Facilities

BIACORE

Clinical Immunology
Laboratory

Myositis Testing

DNA Sequencing

Flow Cytometry

Imaging Core Facility

Microarray Research
Facility

RPA/RNA/In Situ
Hybridization Research
Core

Mouse Genome
Manipulation Facility
(Transgenics)

Signal Transduction
Core Facility

Small Animal MRI

Shared Facilities

Laboratory Animal
Resource Center (LARC)

Institutional Animal
Care and Use
Committee (IACUC)

IN SITU HYBRIDIZATION is an imaging method to visualize mRNA expression in tissues and cells.  A fluorophore-labeled mRNA probe that has consensus sequence to mRNA you are interested in, will be hybridized to the fixed tissues or cells.  The resultant fluorescence will be detected an imaged with a microscope.  Overall procedures include:

1. Synthesis (expression) of mRNA probe

2. Fluorescenceolabeling of the mRNA probe

3. Fixation and hybridization of the specimen

4. Mounting of the specimen and microscopic imaging.

Charges:

Procedures 2-4 (10 slides) for OMRF investigators - $300
Procedures 2-4 (10 slides) for OUHSC investigators - $600
(These charges do not include the cost of synthesis of RNA probe)

Contact Information:
Please contact Dr. Charles Stewart, 271-7583 for details.

Enkekphalin gene expression in mouse brain.

Detailed Information